... (68 °C above) enzyme activity is activated.Mutations induced by recombinant E. coli, after obtained the two separate purified, high purity ... a template, can be a good amplification of DNA fragments 8kb 2,Human genomic DNA as a template, can be a good ... of Taq polymerase inhibition efficiency of 90%Rapid PCR amplification1kb DNA fragment was amplified, only 35 minEfficient PCR amplificationBy hot start ...