... liquid into the EP tube, suck it 8 times by using a transferpettor ( suck to 500µL level ) to mix ... add 600µL wash buffer, and suck it 8 times by using a transferpettor ( suck to 700µL level ) to mix ... add 100µL elute buffer, and suck it 8 times by using a transferpettor ( suck to150µL level ) to mix up ... well. Then put the tube into a constant temperature ( 65°C ) water tank to incubate 10 ...