... 300µL lysis buffer in. Put the tube into a constant temperature ( 65°C ) water tank to incubate ... 15 times to mix up well, leave it in a constant temperature ( 65°C ) water tank for 10min ... extraction of stamen and pistil, this method can work as a reference.For the DNA extraction of plant seeds, ... , and put the embryo without surrounding peristroma into a clean mortar and grind them into thick powder. ...