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One Step Anti-HIV Test
Rapid, Visual Chromatographic Assay for Detection of HIV Antibodies
Advantages:
1. Awarded the No.1 by China CDC during annual HIV Rapid Test Evaluation in 2005&2006&2012.
2. Regularly purchased by WHO & UNICEF
Intended Use:
The advanced quality one step anti-HIV(1&2) tri-line test is a colloidal gold enhanced, rapid immunochromatographic assay for the qualitative detection of antibodies to human immunodeficiency virus(HIV) in human serum or plasma. This test is a screening test. And all positives must be confirmed using an alternate test such s western blot. The test is intended for heal thcare professional use only.
Sample collection&Storage:
1. Collect serum or plasma specimens following regular clinical laboratory procedures.
2. Storage: A specimen should be refrigerated if not used the same day of collection. Specimens should be frozen if not used within 3 days of collecting. Avoid freezing and thawing the specimens more than 2-3 times before using 0.1% of sodium azide can be added to specimen as preservative without affecting the results of the assay.
Features:
Format | Cassette |
Specimen | Whole Blood/Serum/Plasma |
Specificity | Above 99.8% |
Sensitivity | 100% |
Shelf life | 2 years |
Storage | 2-30°C |
Reading Time | 15-20 minutes |
Panel Confirmation | 100% |
Assay Procedure:
1. With the plastic tube to collect blood.
2. Dispense one drop of blood to the “S” well of the test card using the plastic dropper according to the figure.
3. Add two drops of sample diluent to the “D” well immediately after the specimen is added.
4. Interpret test results at 15 minutes.
Performance Characteristics:
1. Specificity In an in-house laboratory study, 63 confirmed negative samples were evaluated with Advanced Quality One Step Anti-HIV(1&2) Tri-line Test using EIA and Western Blot as reference tests. The study gave 100% specificity for the test.
2. Sensitivity In the above-mentioned study, Advanced Quality One Step Anti-HIV(1&2) Tri-line Test was evaluated with 32 confirmed positive samples. The sensitivity of Advanced Quality One Step Anti-HIV(1&2) Tri-line Test was found to be 100% relative to consensus with EIA results, supported by Western Blot assay.
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